bacterial 16s rdna amplicon sequencing  (Novogene)

Journal: Poultry Science

Article Title: Pathogen identification and analysis of broiler bronchial obstruction syndrome using high-throughput sequencing technology

doi: 10.1016/j.psj.2026.107032

Figure Lengend Snippet: Results of 16S rDNA amplification and sequencing of broiler bronchial obstruction specimens. (A)Relative abundance diagrams of dominant bacterial families in the specimens; (B) relative abundance diagrams of dominant bacterial genera; (C) clustering heat map of bacterial family species abundance; (D)clustering heat map of bacterial genus species abundance.

Article Snippet: Bacterial 16S rDNA amplicon sequencing was performed by Beijing Novogene Technology Co., Ltd. (Beijing, China)( ).

Techniques: Amplification, Sequencing

Journal: Microorganisms

Article Title: Metagenomics and Culture Dependent Insights into the Distribution of Firmicutes across Two Different Sample Types Located in the Black Hills Region of South Dakota, USA

doi: 10.3390/microorganisms9010113

Figure Lengend Snippet: Neighbor-joining phylogenetic tree obtained from gyrB rRNA gene sequences of the isolates from the SDLC site. Sequences from this study are shown and isolates closest match from the NCBI database are given in parentheses. The scale bar represents the estimated sequence divergence with an estimated 5000 base substitutions per 1000 nucleotide positions. Percentages refer to significant bootstrap values of 1000 calculated trees. E. coli was used an outgroup.

Article Snippet: The sequencing of bacterial 16S rDNA and gyrB amplicons was done by Molecular Cloning Laboratories (MCLAB, South San Francisco, CA, USA) using the primers, as mentioned earlier.

Techniques: Sequencing

Journal: Microorganisms

Article Title: Metagenomics and Culture Dependent Insights into the Distribution of Firmicutes across Two Different Sample Types Located in the Black Hills Region of South Dakota, USA

doi: 10.3390/microorganisms9010113

Figure Lengend Snippet: Neighbor-joining phylogenetic tree obtained from gyrB rRNA gene sequences of the isolates from the SURF site. Sequences from this study are shown, and isolates closest match from the NCBI database are given in parentheses. The scale bar represents the estimated sequence divergence with an estimated 200 base substitutions per 1000 nucleotide positions. Percentages refer to significant bootstrap values of 1000 calculated trees. E. coli was used as an out group.

Article Snippet: The sequencing of bacterial 16S rDNA and gyrB amplicons was done by Molecular Cloning Laboratories (MCLAB, South San Francisco, CA, USA) using the primers, as mentioned earlier.

Techniques: Sequencing